"Our work focuses on the use of nonlinear optical interactions between femtosecond-duration laser pulses and biological material as a tool for precise ablation of structures and quantitative observation of dynamical processes in live biological samples."

icon_research   FEATURES

Our lab is interested in studying the contribution of multiple physiological systems to disease initiation and progression, with applications in neurodegenerative disease, cardiovascular disease, and cancer.  We would like to understand how the vascular, immune, inflammatory systems and cells native to a tissue interact in these diseases. A major challenge in such work is that model systems such as cell culture or even organotypic tissue culture cannot fully recapitulate all the different cell types involved in disease, so in vivo studies are required. However, it is experimentally difficult to study and manipulate cell-level dynamics in live animals. Recently, we have worked to develop technologies such as improved imaging using multiphoton microscopy that work in whole animals and have sufficient spatial and temporal resolution to quantify cellular dynamics. We also now have tools, femtosecond laser ablation, to produce targeted disruption with cellular-scale precision.

icon_news   NEWS

icon_paper   PUBLICATIONS

Label-free imaging of atherosclerotic plaques using third-harmonic generation microscopy (2018)

Experimentally constrained circuit model of cortical arteriole networks for understanding flow redistribution due to occlusion and neural activation (2017)

Spatio-temporal dynamics of cerebral capillary segments with stalling red blood cells (2017)

In vivo three-photon imaging of activity of GCaMP6-labeled neurons deep in intact mouse brain (2017)

TRAIL-coated leukocytes that prevent the bloodborne metastasis of prostate cancer,” (2016)